For isolation of free-circulating DNA and RNA from human plasma or serum
Concentration of nucleic acids, with high input and low elution volumes
Efficient recovery of fragmented DNA and RNA
No organic extraction or ethanol precipitation
Removal of contaminants and inhibitors
The QIAamp Circulating Nucleic Acid Kit greatly simplifies concentration and purification of free-circulating DNA and RNA from plasma or serum.
The kit can be automated on the QIAcube Connect.
Analysis of tumor-specific extracellular DNA fragments and mRNAs in the blood can enable specific detection of tumor types from a simple blood sample. These circulating nucleic acids are present in serum or plasma usually as short fragments, <1000 bp (DNA) or <1000 nt (RNA). In addition, miRNAs, as small as 21 nt, have the potential to provide biomarkers for certain cancers and disease states.
The QIAamp Circulating Nucleic Acid Kit enables efficient purification of these circulating nucleic acids from human plasma or serum and other cell-free body fluids. Efficient purification with reproducible yields provides a representative population of the circulating nucleic acids in blood (see figure "Reproducible recovery of circulating DNA"). Tube extenders and vacuum processing on the QIAvac 24 Plus enable starting sample volumes of up to 5 ml, and flexible elution volumes between 20 μl and 150 μl allow concentration of nucleic acid species that are present in low concentrations. The kit provides advanced technology of selective binding to a silica-based membrane for improved recovery of fragmented nucleic acids (see figure "Improved recovery of fragmented DNA").