The original Slope Spectroscopy® solution from C Technologies, Inc, the SoloVPE System unlocked the power of Slope Spectroscopy with its unique patented variable pathlength technology. By evolving beyond the limitations of traditional fixed pathlength spectroscopy, the SoloVPE Solution expanded the mature UV-Vis technique from a 2-dimensional to a 3-dimensional science. Conceptually simple, but analytically empowering, C Technologies’ variable pathlength solutions have revolutionized the measurement of concentration by delivering rapid and accurate results while avoiding costly dilution and background correction steps on the widest range of samples which is all made possible by the Slope Spectroscopy technique.
Slope Spectroscopy leverages the power and flexibility of variable pathlength technology to create a rapid, robust, and repeatable concentration measurement method for biologics, small molecules, or any sample typically analyzed with UV-Vis methods. Unlike the single value dependence of legacy UV-Vis methods, the data dense slope method characterizes samples by collecting multiple absorbance data points at several pathlengths to create a section curve (Absorbance vs. Pathlength plot). The section curve is then analyzed in real time to verify linearity in compliance with the Beer-Lambert Law. The linear region of the section curve is directly proportional to the concentration of the sample based upon the sample extinction coefficient. This relationship allows the SoloVPE system to report concentration results in less than 60 seconds.