Research detection kit HTRF

for inflammatory diseasesfor infectious diseasesoncology

The HTRF human IL1 alpha kit is designed for the quantification of human IL1 alpha release in cell supernatant. OverView Also known as hematopoietin 1, IL1 alpha is a pro-inflammatory cytokine mainly produced by activated macrophages, and epilial and endolial cells. Its main role is maintenance and protection of skin's barrier function. In response to infection, IL1 alpha stimulates fibroblast proliferation, increases blood neutrophils, and activates lymphocyte proliferation. Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable detection of low levels of analytes in serum or plasma. HTRF assays offer many advantages over other technologies: Homogeneous add-and-read format No wash steps Low background Straightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates Stable signal, providing flexibility in time of readout or size of assays Assay Principle Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Revvity low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally. Assay data analysis The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve.