Neurological disease detection kit HTRF

for infectious diseasesoncologyimmunity

The HTRF Human IL-2 Detection Kit is an immunoassay designed for the detection and quantitation of Human IL-2 in cell supernatants, in a homogeneous (no-wash steps, no separation steps) format. Overview IL2's major role is to modulate cell-mediated immunity through its effect on T cells. IL2 promotes differentiation of T cells into effector or memory T cells, thus helping body fight off infection. IL2 is also a key player in immuno-oncology since it is main promoter of differentiation of T cells into Treg (regulatory T cells). Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable detection of low levels of analytes in serum or plasma. HTRF assays offer many advantages over other technologies: Homogeneous add-and-read format No wash steps Low background Straightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates Stable signal, providing flexibility in time of readout or size of assays How it works Assay principle Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Cisbio low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.