Research detection kit AlphaLISA™

for antigenslipidfor hepatitis B virus

Hepatitis B surface antigen (HBsAg) is a protein from Hepatitis B virus (HBV). HBV circulates in infected blood when the virus is actively replicating. Infection with HBV induces a spectrum of clinical manifestations and chronic liver diseases. HBsAg can be found on the surface of the lipid envelope containing HBeAg. These antigens are recognized by antibody proteins which bind them. Testing for surface antigens are simple way of detecting the presence of the Hepatitis B virus. The AlphaLISA™ immunoDetection Kit for Hepatitis B Virus Surface antigen (HBsAg) enables the quantitative determination of HBsAg in cell culture media and buffer using a homogeneous AlphaLISA assay (no wash steps). Overview Features: No-wash steps, no separation steps ELISA alternative technology Sensitive detection Broad sample compatibility Small sample volume Results in less than 3 hours Half the time of an ELISA assay AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm. Hepatitis B surface antigen (HBsAg) is a protein from Hepatitis B virus (HBV).