Solution reagent kit BcaBEST™

DNA polymerasefor PCRbacteria

BcaBEST DNA Polymerase ver.2.0 provides a robust, easy solution for rapid, point-of-care pathogen detection via loop-mediate isothermal amplification (LAMP), RT-LAMP, or other isothermal amplification techniques. Originating from the thermophilic bacteria Bacillus caldotenax, BcaBEST DNA Polymerase ver.2.0 has been engineered to enhance the activity of the DNA polymerase without compromising the enzyme’s strong strand-displacement activity, resulting in increased speed and sensitivity. Simply set up your reaction, incubate at 60–65°C for 30 min, and employ your detection method of choice to identify samples containing your desired nucleic acid sequence. BcaBEST DNA Polymerase ver.2.0 is compatible with fluorescent monitoring on a real-time PCR machine and various colorimetric/visual detection methods. BcaBest DNA Polymerase ver 2.0 is a strand-displacing DNA polymerase that has been engineered for increased speed and sensitivity, making it ideal for LAMP, RT-LAMP, and other isothermal PCR applications. BcaBest DNA Polymerase ver.2.0 (8 U/µl) and 2X BcaBEST Buffer containing dNTPs are included. LAMP primers corresponding to a desired nucleic acid sequence are not included and must be provided by the user. Overview Improved sensitivity compared to BcaBEST DNA Polymerase Reaction completes in 30 min or less High thermostability Compatible with real-time fluorescent monitoring and colorimetric/visual detection methods