Infection test kit BacPAK™

viruscellELISA

Accurate baculovirus titering is a vital step in determining the multiplicity of infection (MOI), a critical parameter for achieving optimal and consistent protein yield with baculovirus gene expression. However, traditional methods, such as plaque assays and end-point dilution assays, are laborious and take several days. Our four BacPAK titration solutions are simple, accurate, and less time-consuming than traditional methods. They detect expression of either the baculoviral gp64 protein or p35 protein for quantification. The gp64 protein is expressed during the later stages of baculoviral infection and is only present in viruses capable of infection, while the p35 protein is expressed during the early stages of infection and can be correlated to functional titer. Trying to decide which baculovirus titration product is right for your workflow? Check out the comparison chart below BacPAK Baculovirus GoStix Plus provide a rapid, simple, and effective method for instantly quantifying baculovirus in cell culture supernatants. The test involves applying only 20 µl of precious viral supernatant to a GoStix cassette and waiting 10 minutes for the appearance of test and control bands that indicate the presence of baculoviral gp64. The results on the cassette can then be analyzed using our free smartphone app, which quantifies titer by comparing the intensities of the test and control bands. The quantitative output from the GoStix App (referred to as a GoStix Value, or GV) can be used to compare virus amounts between different preparations in a manner similar to, but far easier and quicker than, an ELISA.