Enzyme reagent kit PicoPLEX® Gold

for DNA sequencingNGS

The PicoPLEX Gold Single Cell DNA-Seq Kit (PicoPLEX Gold) is designed to generate high-quality DNA libraries from single cells for sequencing on Illumina platforms. The kit is based on our patented PicoPLEX technology and is optimized for the reproducible detection of copy number variants (CNVs), single nucleotide variants (SNVs), indels, and small structural variants. The PicoPLEX Gold kit outperforms the leading Multiple Displacement Amplification (MDA) technology for SNV detection. The PicoPLEX Gold Single Cell DNA-Seq Kit has been reconfigured from the original versions of PicoPLEX kits and does not include indexes. PicoPLEX WGA (PicoPLEX) quasi-random priming technology is the international gold standard for whole genome amplification (WGA) for subsequent detection of CNVs in fixed or unfixed single cells. Initially, PicoPLEX chemistry was created for the reproducible detection of aneuploidies and CNVs. To address the need for accurate detection of SNVs, we further optimized the PicoPLEX WGA chemistry using enzymes, primers, and protocols that improve sequencing coverage, uniformity, and accuracy while increasing the resolution for CNVs, SNVs, indels, and other small structural variants while retaining reproducibility. The enhanced chemistry named PicoPLEX Gold is a single-cell library prep kit with a simple, four-step protocol to convert fixed or unfixed single cells into NGS libraries in under three hours with minimum hands-on time. DNA HT Dual Index kits DNA HT Dual Index kits are designed for use with ThruPLEX and PicoPLEX library preparation kits to construct libraries for multiplexed sequencing on Illumina sequencers.