clinical chemistry reagent / serum / alanine aminotransferase / kinetic
for clinical chemistry
ALT (SGPT) Liquid Reagent (A524‐150)
Number of Tests:
500 U/L at 37°C
Normal Range: 3‐35 U/L at 37°C
It is strongly suggested that each laboratory establish its own range of expected values.
Discard if either reagent appears to be cloudy or contains particulate matter.
The working reagent is stable for 2 weeks at 2‐8°C. The working reagent should be
discarded if the initial absorbance, read against distilled water at 340nm, is below 1.000.
Limitations of Procedure:
Hemolysis must be avoided as the concentration of ALT in red cells is roughly 5 times
that of serum. Bilirubin levels up to 40mg/dL and triglyceride levels up to 2000mg/dL
show no interference in this test.