Enzyme reagent kit TUNEL BrightRed

buffer solutionapoptosis

During apoptosis, intracellular specific endonucleases are activated, chromatin DNA is specifically cleaved between nucleosomes, and DNA is degraded into integer multiples of about 180 - 200 bp fragments. The 3'-hydroxyl (3'-OH) end generated by broken DNA can bind to tetramethylrhodamine-deoxyuridine triphosphate (TMR red-dUTP) under the action of terminal deoxynucleotidyl transferase (TdT). Broken DNA labeled with TMR red-dUTP can be directly observed by fluorescence microscopy or quantitatively analyzed by flow cytometry to reflect the level of apoptosis. The BrightRed Labeling Mix contains TMR red-dUTP and Bright factor. This unique small molecule compound can non-covalently bind to TMR red-dUTP, enhance its stability and amplify its signal, resulting in brighter labeled fluorescence and stronger anti-quenching ability. Features Bright factor: Brighter fluorescence, stronger anti-fade ability Additional features: Red fluorescent label for easy co-labeling with other green fluorescents such as GFP Efficient: Highly active recombinant TdT enzyme ensures fluorescence incorporation efficiency Broad compatible: Suitable for cell slides, paraffin sections and frozen sections