Enzyme reagent kit TUNEL BrightGreen

buffer solutionapoptosis

During apoptosis, intracellular specific endonucleases are activated, chromatin DNA is specifically cleaved between nucleosomes, and DNA is degraded into integer multiples of about 180 - 200 bp fragments. The 3'-hydroxyl (3'-OH) end generated by DNA fragmentation can bind to fluorescein-12-deoxyuridine triphosphate (FITC-12-dUTP) under the action of terminal deoxynucleotidyl transferase (TdT). FITC-12-dUTP-labeled DNA can be directly observed by fluorescence microscopy or quantitatively analyzed by flow cytometry to reflect the level of apoptosis. The BrightGreen Labeling Mix contains FITC-12-dUTP and Bright factors. This unique small molecule compound can non-covalently bind to FITC, enhance its stability and amplify its signal, resulting in brighter labeled fluorescence and stronger anti-quenching ability. Features Bright factor: Brighter fluorescence, stronger anti-fade ability Efficient: Highly active recombinant TdT enzyme ensures fluorescence incorporation efficiency Broad compatible: Suitable for cell slides, paraffin sections and frozen sections