Human metapneumovirus reagent kit 7041024

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Vitassay qPCR Kits contains in each well enzymes, buffer, primer and probes as well as an internal control in a lyophilized format. The kits include a positive and a negative control. Identification of pathogen DNA or RNA occurs by the use of target specific primers ans fluorecent labeled hydrolysis probes which hybridize conserved regions in the genomes of the differentpathogens. Real-time PCR kit for the qualitative detection of Metapneumovirus in human samples Intended use Vitassay qPCR Metapneumovirus allows the detection of Metapneumovirus by real-time RT-PCR in clinical samples. The product is intended for use in the diagnosis of Metapneumovirus infections alongside clinical data of the patient and other laboratory tests outcomes. Transport and storage The resuspended positive control should be stored at -20ºC. In order to avoid repeated freeze/thaw cycles, it is recommended to distribute the content in different aliquots. • Keep all reagents in the darkness. Additional equipment and material required • RNA extraction kit • Real-time PCR instrument (thermocycler) (Attached I) • Centrifuge for 1.5 ml tubes • Vortexer • Micropipettes (1-20 µl, 20-200 µl) • Filter tips • Powder-free disposal gloves Summary First identified in 2001, Metapneumovirus is a newly discovered world-wide distributed pathogen, so limited epidemiological data is available. It is known that it causes both upper and lower respiratory tract infections, including bronchiolitis and pneumonia. These infections have been proven to be indistinguishable from the ones caused by RSV and influenza.