RSV reagent kit 7041022

solutionenzymebuffer solution

Vitassay qPCR Kits contains in each well enzymes, buffer, primer and probes as well as an internal control in a lyophilized format. The kits include a positive and a negative control. Identification of pathogen DNA or RNA occurs by the use of target specific primers ans fluorecent labeled hydrolysis probes which hybridize conserved regions in the genomes of the differentpathogens. Real-time PCR kit for the qualitative detection and differentiation of RSV A and B virus in human samples Intended use Vitassay qPCR RSV A + RSV B allows the detection and differentiation of RSV A and RSV B by real-time RT-PCR in clinical samples. The product is intended for use in the diagnosis of RSV infections alongside clinical data of the patient and other laboratory tests outcomes. Transport and storage The resuspended positive control should be stored at -20ºC. In order to avoid repeated freeze/thaw cycles, it is recommended to distribute the content in different aliquots. • Keep all reagents in the darkness. Additional equipment and material required • RNA extraction kit • Real-time PCR instrument (thermocycler) (Attached I) • Centrifuge for 1.5 mL tubes • Vortex • Micropipettes (1-20 µL, 20-200 µL) • Filter tips • Powder-free disposal gloves Summary Human respiratory syncytial viruses (RSV) is the major pathogen responsible for yearly outbreaks of lower respiratory tract disease in young children, but it has also been confirmed as an important cause of respiratory tract illness throughout life. It predictably produces a sizeable outbreak of infections each year.