Membrane ultrafilter Vivaflow® SU TFF Cassettes

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Overview
Vivaflow® SU is a single-use, plug-and-play tangential flow filtration (TFF) cassette for ultrafiltration and diafiltration of intermediate sample volumes (up to 1 L). Designed for R&D and small-scale process development workflows involving proteins, nanoparticles, nucleic acids and viral samples. The housing and flow path have been redesigned and the product is available in an increased range of membrane materials and MWCOs. The lighter housing is produced from renewable, ISCC PLUS certified sources to reduce single-use plastic waste.

Key features

• Suitable for starting volumes up to 1 L and concentration up to 100×
• Multiple membrane materials and MWCOs available (PES, Hydrosart®/HY options; MWCO from 2 kDa to 1,000 kDa and 0.2 µm pore size)
• Plug-and-play single-use cassette format for ultrafiltration and diafiltration workflows
• Reduced single-use plastic waste: housing made from renewable ISCC PLUS certified sources
• Intended for proteins, nanoparticles, nucleic acids and virus-containing samples

Applications

• Cell culture and process fluid clarification
• Protein, nucleic acid and virus concentration
• Nanoparticle separation and enrichment
• Sample volume reduction
• Diafiltration for protein solubilisation and refolding
• Buffer exchange for chromatography sample preparation
• Therapeutic formulation development
• Diafiltration for protein–ligand binding studies

Performance — Time to concentrate 20x (min) at 3 bar feed pressure, 20°C
Table header: Type / Single Device (250 mL start) / Single Device (1 L start) / Solute Recovery Direct / Solute Recovery 10 mL rinse
BSA 1 mg/mL (66 kDa MW):
5 kDa PES / 34 min. / 49 min. / 96% / >99%
10 kDa PES / 22 min. / 32 min. / 94% / >99%
30 kDa PES / 22 min. / 32 min. / 92% / 99%
50 kDa PES / 20 min. / 29 min. / 92% / 98%
γ Globulins 1 mg/mL (150 kDa MW):
100 kDa PES / 43 min. / 62 min. / 92% / 98%
Yeast 1 mg/mL (S. cerevisiae):
0.2 µm PES / 33 min. / 47 min. / 92% / 98%

Ordering examples
Multiple product variants are available (examples of formats and membrane types). Indicative pricing shown at $256.00 per unit. Examples include pore sizes / MWCOs and pack formats (e.g. 2 × 0.1–0.5 L or 1 × 0.5–1.0 L) with product codes (SKUs). Membrane options include PES and Hydrosart® and MWCO range from 2,000 up to 1,000,000 MWCO and 0.2 µm pore size.

Downloads available
Datasheet and manual available for the Vivaflow® SU product line.

Technical specifications

• Dimensions (L × H × W): 107 × 84 × 25 mm
• Channel width × height: 15 × 0.3 mm
• Active membrane surface: 50 cm2
• Minimum recirculation volume: <10 mL
• Non-recoverable hold-up: <0.5 mL
• Materials of construction: Housing – Polycarbonate; Flow channel – Polymethylpentene; Membrane support – Polymethylpentene; Gasket – Silicone; Fittings – Polypropylene or Polyamide; Tubing – Polyvinyl chloride
• Recommended throughput per cassette (rec.): 500 mL
• Recommended flow rate (rec.): 200–400 mL/min
• Maximum pressure at feed port: 3 bar
• Maximum pressure at retentate port: 2.5 bar
• Feed tube run time, maximum: 6 hours
• Operating temperature range: 4–60 °C