Membrane ultrafilter Vivaflow® 200

for liquidsfor protein solutionsfor culture media

Overview
Vivaflow® 200 is a plug-and-play tangential flow filtration (TFF) cassette designed for ultrafiltration and diafiltration of intermediate sample volumes in R&D and process development. Suitable for proteins, nucleic acids, nanoparticles and virus samples. Available in multiple membrane materials and MWCOs to match application requirements. The single-piece cassette eliminates the need for an expensive holder and supports clean-in-place (CIP) for multiple reuses.

Key features

• Up to 5 L starting volume
• Concentrate up to 125×
• Plug-and-play cassette format — single‑piece
• Wide range of membrane materials and MWCOs (PES, Hydrosart® etc.)
• Clean-in-place (CIP) capable for multiple reuses
• No separate cassette holder required
• Minimum recirculation volume <20 mL; non-recoverable hold-up <1.0 mL

Applications

• Cell culture and process fluid clarification
• Protein, nucleic acid and virus concentration
• Nanoparticle separation and enrichment
• Sample volume reduction prior to analysis or formulation
• Diafiltration for protein solubilization and refolding
• Buffer exchange for chromatography sample preparation
• Therapeutic formulation development and small‑scale process evaluation
• Diafiltration for protein–ligand binding studies

Performance (Time to concentrate 20× at 3 bar inlet, 20°C)
Start volume: 1 L — Average flux (mL/min) — Solute recovery Direct / 25 mL rinse

Lysozyme 0.25 mg/mL (14 kDa MW)
2 kDa Hydrosart — 160 min — 6 mL/min — Direct 97% — 25 mL rinse > 99%
3 kDa PES — 180 min — 5 mL/min — Direct 97% — 25 mL rinse > 99%

BSA 1 mg/mL (66 kDa MW)
5 kDa PES — 29 min — 33 mL/min — Direct 98% — 25 mL rinse > 99%
5 kDa Hydrosart — 70 min — 14 mL/min — Direct 98% — 25 mL rinse > 99%
10 kDa PES — 23 min — 41 mL/min — Direct 96% — 25 mL rinse > 99%
10 kDa Hydrosart — 35 min — 27 mL/min — Direct 98% — 25 mL rinse > 99%
30 kDa PES — 25 min — 38 mL/min — Direct 96% — 25 mL rinse 99%
30 kDa Hydrosart — 20 min — 48 mL/min — Direct 96% — 25 mL rinse > 99%
50 kDa PES — 22 min — 43 mL/min — Direct 96% — 25 mL rinse 98%

γ Globulins 1 mg/mL (160 kDa MW)
100 kDa PES — 54 min — 18 mL/min — Direct 96% — 25 mL rinse 99%
100 kDa Hydrosart — 45 min — 21 mL/min — Direct 96% — 25 mL rinse 99%

Yeast 1 mg/mL (S. cerevisiae)
0.2 µm PES — 11 min — 86 mL/min — Direct 92% — 25 mL rinse 98%

Notes on available membrane types / typical product options
Membrane materials and MWCOs include PES (polyethersulfone) and Hydrosart® with MWCOs of 2,000; 3,000; 5,000; 10,000; 30,000; 50,000; 100,000 and pore size 0.2 µm. Multiple SKUs correspond to different membrane types and MWCO combinations (e.g. 0.2 µm PES, 100 kDa HY, 10 kDa PES, 2 kDa HY).

Ordering & typical SKUs
Several single-piece SKUs are available to cover membrane material and MWCO choices. Typical unit price shown on page: $655.00 per cassette (single piece) for selected Vivaflow® 200 variants.

Technical specifications

• Dimensions — Length × Height × Width: 126 × 138 × 38 mm
• Channel width × height: 10 × 0.4 mm
• Active membrane surface: 200 cm²
• Minimum recirculation volume: <20 mL
• Non‑recoverable hold‑up: <1.0 mL
• Housing material: Acrylic
• Flow channel material: Acrylic
• Membrane support: Polyethylene
• Gasket: Silicone
• Fittings: Polypropylene or Polyamide
• Tubing: Polyvinyl chloride (PVC)
• Pressure indicator materials: Polypropylene, Stainless Steel, Polyoxymethylene, Silicone
• Recommended throughput per cassette: 2500 mL
• Recommended flow rate (single cassette): 200–400 mL/min
• Flow rate for two cassettes in parallel: 500–900 mL/min
• Maximum feed port pressure: 4 bar
• Maximum retentate port pressure: 3.5 bar
• Maximum continuous feed run time: 6 hours
• Operating temperature range: 4–60°C