Membrane ultrafilter Vivaflow® 50R

Product overview
Vivaflow® 50R is a single-use, plug-and-play tangential flow filtration (TFF) cassette designed for ultrafiltration and diafiltration of intermediate sample volumes (typical starting volume ≤ 1 L). Active membrane area 50 cm²; compatible with a wide range of membrane materials and MWCOs. Recommended for R&D workflows involving proteins, nucleic acids, nanoparticles and virus-containing samples. Low hold-up volume and simple cleaning-in-place (CIP) enable multiple reuses when validated.

Applications

• Clarification and concentration of cell culture and process fluids
• Concentration and buffer exchange of proteins, nucleic acids and viral particles
• Nanoparticle separation and enrichment
• Sample volume reduction prior to analytical or preparative steps
• Diafiltration for protein solubilization, refolding and formulation screening
• Buffer exchange for chromatography and downstream processing preparation
• Small-scale process development and formulation optimization

Performance (selected data)
Conditions: 3 bar inlet / 2.5 bar outlet, 20°C. Time to concentrate 20× for indicated start volumes and membranes; average flux and solute recovery given as guidance.
Lysozyme 0.25 mg/mL (14 kDa MW): 5 kDa Hydrosart | 70 min | flux 3.4 mL/min | recovery 96% (direct), 98% (25 mL rinse)
Lysozyme 0.25 mg/mL: 10 kDa Hydrosart | 23 min | flux 10.3 mL/min | recovery 94%, 96% (25 mL rinse)
BSA 1 mg/mL (66 kDa MW): 10 kDa Hydrosart | 24 min | flux 9.9 mL/min | recovery 98%, >99% (25 mL rinse)
BSA 1 mg/mL: 30 kDa Hydrosart | 15 min | flux 15.8 mL/min | recovery 97%, >99%
γ-Globulins 1 mg/mL (150 kDa MW): 100 kDa Hydrosart | 46 min | flux 5.2 mL/min | recovery 97%, >99%
Start volume 1 L (one Vivaflow® 50R), 10 kDa Hydrosart: BSA 1 mg/mL | 95 min | flux 10.0 mL/min | recovery 98%, >99%
Start volume 1 L (two Vivaflow® 50R in parallel), 10 kDa Hydrosart: BSA 1 mg/mL | 48 min | flux 19.8 mL/min | recovery 98%, >99%

Technical specifications (summary)

• Model: Vivaflow® 50R
• Dimensions (L × H × W): 100 × 100 × 24 mm
• Channel width × height: 7.5 × 0.4 mm
• Active membrane surface: 50 cm²
• Minimum recirculation volume: 10 mL
• Non-recoverable hold-up volume: < 0.5 mL
• Recommended throughput per cassette: 500 mL
• Recommended flow rate: 200–400 mL/min
• Maximum pressures: feed port 4 bar; retentate port 3.5 bar
• Maximum continuous feed tube run: 6 hours
• Operating temperature range: 4–60°C
• Materials: housing & flow channel — acrylic; membrane support — polyethylene; gasket — silicone; fittings — polypropylene or polyamide; tubing — PVC

Ordering and variants
Multiple MWCO and membrane material variants available (examples: 5 kDa, 10 kDa, 30 kDa, 100 kDa Hydrosart). Typical pack size: 1 piece. Contact supplier for pricing, lead times and validated membrane options for specific applications.

Downloads
Datasheet and user manual (PDF) available on the product page—see downloads section for technical drawings and validation data.

Equipment required
Peristaltic pump with compatible pump head and tubing, sample/diafiltration reservoir, pressure indicator and appropriate fittings. Refer to the user manual for recommended setup, flow rate ranges and cleaning procedures.