Membrane filter Sartobind® Chromatography Adsorbers

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Overview
The Sartobind® membrane adsorbers are syringe‑filter format membrane devices for ion‑exchange and affinity chromatography. Ligands are covalently bound to the internal microporous membrane surfaces, delivering high binding capacities while the membrane pore structure enables rapid mass transfer and high flow rates. These single‑use capable devices eliminate column packing steps and are available for multiple reuses depending on process conditions. Offered in sizes with bed volumes from 0.4 to 2.8 mL.

Key features

• Format: syringe‑filter style membrane adsorber
• Bed volumes: 0.4 to 2.8 mL (multiple sizes)
• Rapid mass transfer and fast flow rates due to microporous membrane structure
• Ligands covalently bound to membrane surfaces for high binding capacity
• Compatible with repeated use depending on cleaning/regeneration protocol

Applications

• Purification of plasmid DNA and oligonucleotides
• Protein and peptide purification
• Purification of viruses and viral vectors
• Enrichment of nanoparticles and extracellular vesicles
• Removal of leached Protein A
• Purification of monoclonal antibodies (mAbs) and antibody‑drug conjugates (ADCs)

Technical specifications

• Membrane adsorber types: Sartobind S, Sartobind Q, Sartobind D
• Ligand density: Sartobind S/Q = 2–5 µEq/cm²; Sartobind D = 2–4 µEq/cm²
• Ligand type: S = strong acidic cation; Q = strong basic anion; D = weak basic anion
• Functional group: S = sulphonic acid; Q = quaternary ammonium; D = diethylamine
• pH stability (cleaning & regeneration): S = 3–14; Q = 2–14; D = 2–14
• pH stability (overnight & longer): S = 4–13; Q = 2–12; D = 4–13
• Approximate pKa: S = 1; Q = 11; D = 9.5
• Capacities & dimensions (S/Q/D — 0.4 mL): Height 25 mm; Housing Ø 36 mm; Membrane Ø 25 mm; Binding capacity* 10.5–12 mg/unit; Membrane area 15 cm²; Nominal pore size >3 µm; Frontal surface area 5 cm²; Number of layers 3; Bed height 0.8 mm
• Capacities & dimensions (S/Q/D — 2.1 mL): Height 28 mm; Housing Ø 36 mm; Membrane Ø 25 mm; Binding capacity* 52.5–60 mg/unit; Membrane area 75 cm²; Nominal pore size >3 µm; Frontal surface area 5 cm²; Number of layers 15; Bed height 4.0 mm
• Capacities & dimensions (S/Q/D — 2.8 mL): Height 31 mm; Housing Ø 66 mm; Membrane Ø 50 mm; Binding capacity* 70–80 mg/unit; Membrane area 100 cm²; Nominal pore size >3 µm; Frontal surface area 10 cm²; Number of layers 5; Bed height 1.4 mm
• Materials: Housing = polypropylene; Protective caps = polycarbonate
• Note: *See Sartobind® manual for detailed binding capacity data and recommended cleaning/regeneration procedures