Ion chromatography column Vivapure® Ion Exchange Spin Columns

for researchfor protein purificationhigh-capacity

Product overview The Vivapure® ion exchange spin columns are centrifugal-format membrane adsorbers for ion-exchange chromatography. Ligands are covalently bound to internal microporous surfaces to provide high binding capacities. The membrane pore structure enables rapid mass transfer and fast sample flow rates. Columns are supplied in Mini and Maxi formats and are suitable for multiple reuses. Bed volume range: 0.24 to 2.7 mL.

Downloads (available) Data Sheet; Manual; Lab Chromatography Brochure.

Applications

• Cell-culture and process-fluid clarification
• Protein, nucleic acid and virus concentration
• Nanoparticle separation and enrichment
• Sample volume reduction
• Diafiltration for protein solubilization and refolding
• Buffer exchange for chromatography sample preparation
• Therapeutic formulation development
• Diafiltration for protein–ligand binding studies

Ordering examples (selected items shown on page)

• Vivapure D Maxi H, 8pc — $526.00
• Vivapure D Mini H, 24pc — $278.00
• Vivapure Q Maxi H, 8pc — $526.00
• Vivapure Q Mini H, 24pc — $278.00
• Vivapure S Maxi H, 8pc — $526.00
• Vivapure S Mini H, 24pc — $278.00

Technical specifications (table) Membrane adsorber — Nominal pore size: 3–5 µm (S, Q, D)
Thickness: 230–320 µm (S, Q, D)
Ligand density: 145–218 µEq/mL (S, Q, D)
Ligand type: Vivapure S: strong acidic cation; Vivapure Q: strong basic anion; Vivapure D: weak basic anion
Functional group: S: sulphonic acid; Q: quaternary ammonium; D: diethylamine
Working pH: Vivapure S/Q: 2–12; Vivapure D: 4–10
Approximate pKa: S: 1; Q: 11; D: 9.5

Capacities & Dimensions Sample volume (fixed-angle rotor): Mini <0.4 mL; Maxi <10.5 mL
Sample volume (swing-out rotor): Mini N/A; Maxi <19 mL
Binding capacity*: Mini ≈4 mg; Maxi ≈60–80 mg
Membrane area: Mini 7.48 cm2; Maxi 84.4 cm2
Bed volume: Mini 0.24 mL; Maxi 2.7 mL

Materials of Construction Housing: polypropylene
Membrane support: stabilized regenerated cellulose

* Binding capacities established using 1 mg/mL BSA in 25 mM Tris-HCl (pH 8) for Vivapure Q and D devices, or 1 mg/mL cytochrome c in 25 mM sodium acetate buffer (pH 5.5) for Vivapure S devices. Actual capacities depend on the target molecule and buffer conditions.

Features / technical specifications

• Bed volume: 0.24 mL (Mini) to 2.7 mL (Maxi)
• Membrane nominal pore size: 3–5 µm
• Membrane thickness: 230–320 µm
• Ligand density: 145–218 µEq/mL
• Ligand types: strong acidic cation (S), strong basic anion (Q), weak basic anion (D)
• Functional groups: sulphonic acid (S), quaternary ammonium (Q), diethylamine (D)
• Working pH range: S/Q = 2–12; D = 4–10
• Approximate pKa values: S = 1; Q = 11; D = 9.5
• Binding capacities (approx.): Mini ≈4 mg; Maxi ≈60–80 mg (dependent on molecule and buffer)
• Membrane area: Mini 7.48 cm²; Maxi 84.4 cm²
• Housing material: polypropylene; membrane support: stabilized regenerated cellulose