Research chromatography column Vivapure® Viral Vector Kits

high-capacity

Product Overview
The Vivapure® Adenopack and Lentiselect kits are complete kits for purification of adenovirus type 5 and VSV-G pseudotyped lentivirus. They support processing of sample volumes up to 1,000 mL using membrane adsorber technology for high binding capacity and faster flow rates. Kits include filters, membrane chromatography units and buffers for faster separations compared with CsCl density gradients or conventional ultracentrifugation.

Applications

• Purification of adenovirus type 5 (Ad5)
• Purification of VSV-G pseudotyped lentivirus

Performance — Purification of Ad5-GFP Constructs (Adenopack 20, 100, 500)

• Adenopack 20 — Sample volume: 20 mL; Process time: 1 hr; Eluate: 1 mL; Recovery: 65–70%; Viral particles: ~1 × 10^11–10^12 VP
• Adenopack 100 — Sample volume: 60 mL; Process time: 1–2 hr; Eluate: 1 mL; Recovery: ~65%; Viral particles: ~1–3 × 10^12 VP
• Adenopack 100 (200 mL test) — Sample volume: 200 mL; Process time: 2 hr; Eluate: 1 mL; Recovery: ~80%; Viral particles: ~1 × 10^13 VP
• Adenopack 500 — Sample volume: 500 mL; Process time: 2 hr; Eluate: 1 mL; Recovery: ~80%; Viral particles: ~1–3 × 10^13 VP
• Comparator — CsCl density gradient: 500 mL; Process time: 24–48 hr; Eluate: 1–2 mL (after dialysis); Recovery: 60–70%; Viral particles: ~1 × 10^11–10^12 VP

Performance — Purification of VSV-G Pseudotyped Lentivirus Constructs (Lentiselect 40, 500, 1000)

• Lentiselect 40 — Sample volume: 40 mL; Process time: 45 min; Eluate: 0.2 mL (after desalting); Recovery: ~50%; Infective particles: ~8 × 10^8 IP
• Lentiselect 500 — Sample volume: 500 mL; Process time: 3 hr; Eluate: 1 mL (after desalting); Recovery: ~35%; Infective particles: ~2–5 × 10^9 IP
• Lentiselect 1000 — Sample volume: 1,000 mL; Process time: 6 hr; Eluate: 2 mL (after desalting); Recovery: ~35%; Infective particles: ~1 × 10^10 IP
• Comparator — Ultracentrifugation: 500 mL; Process time: 10–11 hr; Eluate: 0.5 mL; Recovery: ~25%; Infective particles: ~3 × 10^9 IP

Technical specifications — summary

• Vivapure® Adenopack Kits (Adenopack 20 / 100 / 500): Number of purifications — 6 × 20 mL / 2 × 20–60 mL or 1 × 200 mL / 1 × 500 mL; Typical virus titre (VP/mL) — 1 × 10^11–1 × 10^12 / ~1 × 10^13 / ~3 × 10^13; VP/PFU ratio — 50–100 / 20–50 / 20–50; Typical processing time — 1 hr / 2 hr / 2 hr; Endotoxin level — < 0.025 EU/mL
• Vivapure® Lentiselect Kits (Lentiselect 40 / 100 / 500 / 1000 as applicable): Number of purifications — 4 × 40 mL / 1 × 500 mL / 1 × 1,000 mL; Typical infective particles (IP/mL) — ~3 × 10^9 / 2–5 × 10^9 / 4–5 × 10^9; Final eluate volume — 0.2 mL / 1 mL / 2 mL; VP/PFU ratio — 5–15 / 20–50 / 20–50; Typical processing time — 45 min / 3 hr / 6 hr; Endotoxin level — < 0.025 EU/mL
• Contents included: filters, membrane adsorber chromatography units and buffers
• Accessories available: pump heads (1.6 mm, 2.4 mm), pump (110–240 V), tubing sets, adapter kit for lab LC systems

Downloads (available documents)

• Data Sheet — Vivapure Adenopack and Lentiselect data sheet
• Manual — Vivapure® Adenopack 20
• Manual — Vivapure® Adenopack 20 RT
• Manual — Vivapure® Adenopack 100
• Manual — Vivapure® Adenopack 100 RT
• Manual — Vivapure® Adenopack 500
• Manual — Vivapure® Adenopack 500 RT
• Manual — Vivapure® Lentiselect 40
• Manual — Vivapure® Lentiselect 500
• Manual — Vivapure® Lentiselect 1000
• Lab Chromatography Brochure

Equipment Required
Refer to the product manuals for required equipment and pump/tubing specifications (see manuals listed above).

Ordering / Available kit options & accessories (summary)

• Example SKUs & pricing (USD): Vivapure Adenopack 100 RT (VS-AVPQ102) $598.00; Adenopack 100 with Benzonase (VS-AVPQ101) $806.00; Adenopack 20 RT (VS-AVPQ022) $335.00; Adenopack 20 with Benzonase (VS-AVPQ020) $449.00; Adenopack 500 RT (VS-AVPQ502) $1,957.00; Adenopack 500 with Benzonase (VS-AVPQ501) $1,762.00; Vivapure Lentiselect 1000 (VS-LVPQ1000) $2,843.00; Lentiselect 40 (VS-LVPQ040) $867.00; Lentiselect 500 (VS-LVPQ500) $1,708.00
• Accessories: peristaltic pump heads, pump tubing sets, peristaltic pump, Sartobind Lab LC system adapter kits

Features / Technical specifications

• Product family: Vivapure® Viral Vector Kits (Adenopack and Lentiselect)
• Max sample volume: up to 1,000 mL (Lentiselect 1000)
• Final eluate volumes: Adenopack typically 1 mL; Lentiselect 0.2–2 mL depending on kit
• Typical recoveries: Adenopack 65–80% depending on kit; Lentiselect ~35–50% depending on kit
• Typical virus titres achievable: Adenopack up to ~1 × 10^13–3 × 10^13 VP/mL (kit dependent)
• Typical infective particle concentrations (Lentiselect): 2–5 × 10^9 IP/mL (kit dependent)
• VP/PFU ratios: Adenopack 20–100; Lentiselect 5–50 depending on kit
• Typical processing times: Adenopack 1–2 hr; Lentiselect 45 min to 6 hr (kit dependent)
• Endotoxin level: < 0.025 EU/mL across kits
• Contents: filters, membrane adsorber chromatography units and buffers included
• Accessories available: pump heads (1.6 mm, 2.4 mm), pump (110–240 V), tubing sets and adapter kit for lab LC systems