Enzyme reagent Degradase Plus

for microbiologyfor nucleic acidsphosphate

Quick and simple procedure for generating single nucleosides from DNA for quantitative analysis via LC/MS Convienent 2 hour, single enzyme digest v. conventional 6-16 hr multi-step enzyme digestion protocol DESCRIPTION DNA Degradase Plus from Zymo Research is a nuclease mix that quickly and efficiently degrades DNA to its individual nucleoside components. Since nucleosides lack negatively charged phosphate, DNA Degradase Plus is ideal for whole-genome DNA methylation analysis by LC/MS. Digestion with the enzyme is performed via a one-step procedure that is faster and simpler than other available methods. TECHNICAL SPECIFICATIONS Assay ConditionDNA Degradase Plus in 1X DNA Degradase Reaction Buffer. Incubate reaction mixtures at 37C for ≥1 hour Concentration5 U/µl Enzyme InactivationHeat inactivate enzyme at 70C for 20 minutes Unit Definition5 U of enzyme will digest 1 µg of genomic DNA in 25 µl reaction volume at 37C for ≥ 1 hr