SELL YOUR PRODUCTS
*mymedicalexpo  
English

Bisulfite conversion reagent kit D5004 / D5003
for nucleic acidssodium

bisulfite conversion reagent kit
bisulfite conversion reagent kit
Add to favorites
Compare this product
 

Characteristics

Applications
for bisulfite conversion, for nucleic acids
Tested parameter
sodium

Description

HIGHLIGHTS Streamlined, proven procedure for bisulfite conversion of DNA. Desulphonation and recovery of bisulfite-treated DNA with a 96-well spin-column plate. Recovered DNA is ideal for downstream analyses including PCR, endonuclease digestion, sequencing, microarrays, etc. DESCRIPTION The EZ-96 DNA Methylation Kits are bisulfite conversion kits that feature a high-throughput (96-well spin-plate), simplified procedure that streamlines bisulfite conversion of DNA. Cytosines undergo a three-step reaction with sodium bisulfite during which the cytosine is converted into uracil. The innovative in-column desulphonation technology eliminates otherwise cumbersome precipitations. The kit is designed to reduce template degradation and minimize DNA loss during treatment and clean-up, while ensuring complete conversion of the DNA. Purified, converted DNA is ideal for downstream analyses including PCR amplification, endonuclease digestion, sequencing, microarrays, etc. Note: Catalog # D5004 is recommended for use with the Illumina Infinium MethylationEPIC BeadChip array. TECHNICAL SPECIFICATIONS ApplicationsPurified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-generation sequencing, PCR amplification, etc. Catalog # D5004 is recommended for use with Illumina Infinium MethylationEPIC BeadChip array. Conversion>99% Elution Volume≥ 15 µl for Deep-well ≥ 30 µl for Shallow-well EquipmentThermocycler with heated lid, swinging-bucket centrifuge with plate carriers. Input500 pg - 2 µg of DNA Processing Time12-16 hours

Catalogs

No catalogs are available for this product.

See all of Zymo Research‘s catalogs
*Prices are pre-tax. They exclude delivery charges and customs duties and do not include additional charges for installation or activation options. Prices are indicative only and may vary by country, with changes to the cost of raw materials and exchange rates.