LINEAR CHEMICALS diagnostic reagents

Storage temperature: 2 °C - 8 °C

IFCC UV enzymatic method KINETIC PRINCIPLE Aspartate aminotransferase (AST/GOT) catalyzes the transfer of the amino group from aspartate to oxoglutarate with the formation of glutamate and oxalacetate. The latter is reduced to malate ...

Storage temperature: 2 °C - 8 °C

TOTAL and DIRECT Colorimetric method END POINT PRINCIPLE Bilirubin is converted to coloured azobilirubin by diazotized sulfanilic acid and is measured photometrically. Of the two bilirubin fractions in serum –bilirubin-glucuronide ...

Storage temperature: 2 °C - 8 °C

DIRECT DPD – Colorimetric method END POINT WITH SAMPLE BLANK PRINCIPLE Direct bilirubin (conjugated) reacts with the diazonium salt 2,4-dichlorophenyldiazonium (2,4-DPD) in the presence of sulfamic acid to form azo-bilirubin, this ...

Storage temperature: 2 °C - 8 °C

TOTAL Colorimetric method ENDPOINT PRINCIPLE The method is based on the specific binding of arsenazo III and calcium at acid pH with the resulting shift in the absorbtion wavelength of the complex. The intensity of the cromophore ...

Storage temperature: 2 °C - 8 °C

TOTAL Colorimetric method ENDPOINT PRINCIPLE The method is based on the specific binding of cresolftalein complexone (OCC), a metallochromic indicator, and calcium at alkaline pH with the resulting shift in the absorbtion wavelength ...

Storage temperature: 2 °C - 8 °C

THIOCYANATE Colorimetric method ENDPOINT PRINCIPLE Chloride ions in the sample quantitatively displaces thiocyanate from mercuric thiocyanate. Liberated thiocyanate ion reacts with ferric ion forming a red ferric-thiocyanate complex ...

Storage temperature: 2 °C - 8 °C

TOTAL Enzymatic colorimetric method ENDPOINT PRINCIPLE This method for the measurement of total cholesterol in serum involves the use of three enzymes: cholesterol esterase (CE), cholesterol oxidase (CO) and peroxidase (POD). In ...

Storage temperature: 2 °C - 8 °C

TOTAL AND INHIBITED Enzymatic colorimetric method KINETIC PRINCIPLE Cholinesterase (CHE) catalyzes the hydrolysis of butyrylthiocholine substrate forming butyrate and thiocholine. The latter reduces 5,5´- mercaptobis-2-nitrobenzoic ...

Storage temperature: 2 °C - 8 °C

CK NAC-ACTIVATED UV enzymatic method KINETIC PRINCIPLE Creatine kinase (CK) catalyzes the reaction between creatine phosphate (CP) and adenosine 5´-diphosphate (ADP) with formation of creatine and adenosine 5´-triphosphate (ATP). ...

Storage temperature: 2 °C - 8 °C

CK –MB INHIBITED Immunological UV method KINETIC PRINCIPLE The major CK activity in normal serum is due to CK-MM and CKMB isoenzymes, found mainly in skeletal and heart muscle. CK-BB is ussualy present in serum at low concentration. Both, ...