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Solution reagent AST/GOT BR
diagnosticliquidfor AST

solution reagent

solution reagent

solution reagent

solution reagent

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Characteristics

Type

solution

Applications

diagnostic

Format

liquid

Tested parameter

for AST

Method

enzymatic

Storage temperature

Max.: 8 °C
(46 °F)

Min.: 2 °C
(36 °F)

Description

IFCC UV enzymatic method KINETIC PRINCIPLE Aspartate aminotransferase (AST/GOT) catalyzes the transfer of the amino group from aspartate to oxoglutarate with the formation of glutamate and oxalacetate. The latter is reduced to malate by malate dehydrogenase (MDH) in the presence of reduced nicotinamide adenine dinucleotide (NADH). The reaction is monitored kinetically at 340 nm by the rate of decrease in absorbance resulting from the oxidation of NADH to NAD+, proportional to the activity of AST present in the sample.

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