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Solution reagent kit KR10082
diagnosticliquidDPD

Solution reagent kit - KR10082 - LINEAR CHEMICALS - diagnostic / liquid / DPD

Solution reagent kit - KR10082 - LINEAR CHEMICALS - diagnostic / liquid / DPD - image - 2

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Characteristics

Type

solution

Applications

diagnostic

Format

liquid

Tested parameter

DPD, bilirubin

Method

colorimetric

Storage temperature

Max.: 8 °C
(46 °F)

Min.: 2 °C
(36 °F)

Description

Diazotized sulfanilic acid transforms bilirubin into colored azobilirubin, which is determined photometrically. Of the two fractions of bilirubin present in serum, bilirubin glucuronate and bilirubin free associated with albumin, only the first reacts directly, while the free bilirubin needs to be dissociated from the protein by an accelerator to react. Indirect bilirubin is calculated by the difference between total bilirubin (with accelerator) and direct bilirubin (without accelerator). The concepts of 'direct' and 'indirect' refer exclusively to the reaction characteristics in the presence or absence of accelerators or solubilizers and are only approximate equivalents to the two mentioned bilirubin fractions. Direct (conjugated) bilirubin reacts with the diazonium salt 2,4-dichlorophenyldiazonium (2,4-DPD) in the presence of sulfamic acid, forming azobilirubin. This colored complex can be measured photometrically at 546 nm. Of the two fractions of bilirubin present in serum, bilirubin glucuronate (conjugated) and bilirubin free associated with albumin (unconjugated), only the first reacts directly, while the free bilirubin needs to be dissociated from the protein by an accelerator to react. Indirect bilirubin is calculated by the difference between total bilirubin (with accelerator) and direct bilirubin (without accelerator). The concepts of 'direct' and 'indirect' refer exclusively to the reaction characteristics in the presence or absence of accelerators or solubilizers and are only approximate equivalents to the two mentioned bilirubin fractions.

Catalogs

General Catalogue

36 Pages

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