Agarose gel reagent Load Ready™

dyefor PCRliquid

A ready-to-use Master Mix containing all the reagents needed for a successful PCR experiment – just add your template DNA and primers! EZ PCR Master Mix is Load Ready™, meaning it may be loaded directly onto an agarose gel after completion of PCR, without the need to add a loading dye. It includes two tracking dyes for easy electrophoresis monitoring. 240 μl vial Sufficient for 60 reactions at 4 μl master mix per reaction (i.e., 20 μl total reaction volume) Stable formulation may be shipped or temporarily stored for up to 1 month at room temperature, or up to 1 year at -20°C TECHNICAL SPECIFICATIONS Composition: Taq DNA polymerase 5X reaction buffer (0.4 M Tris-HCl, 0.1 M (NH4)2SO4, 0.1% w/v Tween-20) 7.5 mM MgCl2 (in 1X PCR solution: 1.5 mM MgCl2) 1 mM each dNTP (in 1X PCR solution: 200 μM dATP, 200 μM dCTP, 200 μM dGTP, 200 μM dTTP) Blue dye with migration rate equivalent to 3.5-4.5 kb DNA fragment Yellow dye with migration rate in excess of primers in 1% agarose gel Compound that increases sample density for direct gel loading May be shipped or temporarily stored for up to 1 month at room temperature or for up to 6 months at 2-8°C without discernible loss of activity