Solution reagent kit

for real-time PCRfor RT-PCRRT-qPCR

The COVID-19 Real Time RT-PCR Kit is a real-time reverse transcription polymerase chain reaction (rRT-PCR) test for the qualitative detection of ORF1ab and N gene from COVID-19 in upper and lower respiratory specimens (nasopharyngeal or oropharyngeal swabs,sputum, BALF, etc.) collected from individuals suspected of COVID-19 by their healthcare provider. Components: 1.SARS-COV-2 reaction mixture, lyophilized;Primers, probes, PCR reaction buffer, dNTPs, Enzyme, etc. 2.SARS-COV-2 positive control, lyophilized；Lyophilized pseudoviral particles including ORF1ab and N genes detecting target sequence 3.Ultrapure water(negative control);Ultrapure water. Features: Lyophilized RT-PCR Pre-mixture：No cold-chain transportation required. Detection targets: ORF1ab/N gene. Quality control: Endogenous internal standard is a quality control for sample collection, transportation, nucleic acid extraction and detection. Accuracy: The results of enterprise's reference products detected by this kit are correct. Specificity:No cross-reaction with various vivuses,H1N1,influenza B virus,respiratory syncytical virus,etc. Precision:For 10,000 copies/mL and 2,000 copies/mL(Simulation samples or nucleic acids),Inter-assay CV≤5%,Intra-assay CV≤5%. Interference test:The possible endogenous interfering substances have no obvious interference to this kit,such as 30% blood and 10mg/mL much.