Nucleic acid reagent COT I

for hybridizationFISHfor blocking

Short description
COT I Human DNA is prepared from male human placental genomic DNA and is used as blocking DNA to reduce background and cross-hybridization in microarray and in situ hybridization assays.

Product details
BIORON supplies COT I Human DNA, produced in Germany. The COT fraction is obtained by controlled fragmentation of male placental genomic DNA. The manufacturing process ensures a consistent size distribution predominantly between 50 and 300 base pairs, optimizing blocking performance for repetitive sequences. Custom specifications are available on request.

Characteristics

• Balanced proportion of Y‑chromosomal sequences
• Enriched in fast-annealing repetitive elements
• Consistent size distribution: 50–300 bp
• Customizable for production requirements

Tested quality

• Concentration assessed by photometric and fluorometric methods
• Purity A260/280 > 1.6

Highest safety

• Phenol treated
• Tested for HIV I/II, HCV and HBV

Applications

• Filter and microarray hybridization assays
• In situ hybridization (e.g., FISH)
• Single-copy gene hybridization
• Reduction of cross-hybridization and background signal

Pricing / Product variants
COT I Human DNA — photometric
REF: 402007
Concentration (photometric): 1 – 2 mg/ml
Pack size: 500 µg

COT I Human DNA — fluorometric
REF: 402011
Concentration (fluorometric): 1.1 – 1.5 mg/ml
Pack size: 1 ml

Technical specifications

• Commercial name: COT I Human DNA
• Source: male human placental genomic DNA
• Fraction: COT fraction obtained by fragmentation
• Main composition: predominantly fast-annealing repetitive elements
• Size distribution: 50–300 base pairs
• Concentration (photometric): 1 – 2 mg/ml
• Concentration (fluorometric): 1.1 – 1.5 mg/ml
• Pack sizes: 500 µg (photometric), 1 ml (fluorometric)
• REF / SKU: 402007 (photometric), 402011 (fluorometric)
• Purity: A260/280 > 1.6
• Quality control: tested by photometric and fluorometric methods
• Safety: phenol treated; tested for HIV I/II, HCV and HBV
• Made in Germany
• Customizable product specifications available