Nucleic acid reagent

proteinase Kfor hybridizationfor DNA analysis

Overview
Human Placenta DNA is supplied as a liquid extract, purified exclusively from human placental tissue of a male newborn. The material is treated with Proteinase K followed by phenol extraction and precipitation. The DNA exhibits high molecular weight with fragment size > 10 kb and is tested negative for HIV, HCV and HPV. This preparation is suitable as a blocker of human repetitive sequences and for hybridization-based applications. BIORON also offers COT I Human DNA (enriched small repetitive sequences of placenta DNA).

Applications

• Genomic analysis
• Construction of genomic libraries
• FISH (fluorescent in situ hybridization)
• Southern and Northern hybridization techniques

Additional information
Product ID: human-placenta
Pack size: 1 mg

Technical characteristics / specifications

• Source: human placental tissue (male newborn)
• Format: liquid
• Treatment / purification: Proteinase K treatment; phenol extraction and precipitation
• Fragment size: > 10 kb
• Molecular weight / purity: high molecular weight; high purity
• Pathogen testing: tested for absence of HIV, HCV and HPV
• Functional note: specifically blocks human random repeat sequences; ideal blocker for hybridization experiments (FISH, Southern, Northern)
• Applications: genomic analysis; construction of genomic libraries; hybridization techniques
• Available pack sizes: small packs and bulk (prices on request)
• Pack size shown on page: 1 mg
• SKU / REF: N/A