DNA polymerase reagent GreenHot

dNTP solutionbuffer solutionfor qPCR

Short description
Ultimate GreenHot Master Mix is a 2x concentrated, ready-to-use liquid master mix formulated for qPCR and multiplex applications. It contains HotStart polymerase, balanced dNTPs, optimized MgCl2 levels and SYBR Green dye for fluorescence-based detection.

Product details
The kit is supplied as a liquid 2x master mix and includes additional PCR-grade water and MgCl2. Components: HotStart DNA polymerase, PCR buffer, dNTPs, MgCl2 and SYBR Green dye. Only primers and template DNA need to be added. The HotStart formulation reduces non-specific amplification and simplifies setup. The mix is compatible with SYBR Green assays and configured for high-performance qPCR and multiplex workflows. Short-term stability at room temperature (+18 to +25 °C) is up to two weeks; for long-term storage keep at -20 °C.

Applications

• Standard PCR
• Real-time qPCR (SYBR Green detection)
• Pathogen detection and diagnostics
• Multiplex PCR assays

Standard Protocol (indicative)
Step: Initial denaturing | Time: 1 – 3 min | Temperature: 92°C – 95°C | Cycle: 1 x
Step: Denaturing | Time: 5 – 10 sec | Temperature: 92°C – 95°C | Cycle: per protocol
Step: Combined annealing & elongation | Time: 10 – 20 sec | Temperature: 60°C or primer-dependent | Cycle: 25 – 40 x

Technical specifications

• Denomination: Ultimate GreenHot Master Mix
• Formulation: 2x concentrated, liquid, ready-to-use
• Key components: HotStart Polymerase, PCR buffer, dNTPs, MgCl2, SYBR Green Dye
• Set contents: Master Mix plus additional PCR grade water and MgCl2 included
• Optimized for: qPCR and multiplex applications
• Applications: Standard PCR, Real-Time PCR, Pathogen detection, Multiplex applications
• Stability: Stable at +18 to +25 °C for up to two weeks; long-term storage at -20 °C
• Available pack sizes: 2×100 rcs, 10×100 rcs
• Concentration: 2x