DNA polymerase reagent kit pColiExpress™ I

ampicillinDNA ligasefor PCR

For a highly Efficient, Fast & Versatile Protein Expression (T4 DNA Ligase based) in E. coli from PCR fragment cloned Product Overview: pColiExpress™ I Cloning & Expression Kit is a DNA Cloning System for Protein Expression in E.coli. pColiExpress™ technology allows efficiently cloning DNA fragments and the quick production of a large quantity of any desired Protein. pColiExpress™ I contains all elements required for expression of large quantity of any desired protein by T7 RNA Polymerase Inducible System and a His6 tag at NH2 end that allow the protein purification. The vector has also an f1 origin of replication, an ampicillin resistance cassette, and a pBR322 origin of replication. Advantages & Features Ready-to-use vectors. Highly efficient cloning system. Special design that allows the cell to house larger numbers of copies than other plasmids with ori pBR322. Linearized vector: ready for ligation with your PCR amplified with the recommended primers. Minimum background: lower than 1%. Time-saving protocol: avoids any step required after PCR. Higher protein expression than other suppliers. Cost avoidance: avoids the use of expensive phosphorylated primers. Versatility: cloning of PCR fragments amplified with any type of DNA Polymerase. Risk-free: product covered by our Quality 100% Guarantee. Includes Includes for 20 rxn: – 20 µL pColiExpress™ linearized vector (50 ng/µL) – 200 µL T4 DNA Ligase Buffer (5x) – 20 µL T4 DNA Ligase (5 U/µL) – 10 µL Control Insert DNA (30 ng/µL) – 5 µL pColiExpress™ Control (50 ng/µL) Plus Version Includes for 20 rxn – 20 µL pColiExpress™ linearized vector (50 ng/µL) – 200 µL T4 DNA Ligase Buffer (5x)