Gelatine reagent PhotoGel®

for researchlyophilizedpowder

Short description
PhotoGel® 50% DS is a sterile, lyophilized methacrylated gelatin (GelMA) with a medium degree of methacrylation. It is a freeze-dried porcine gelatin functionalized with methacrylate groups. This GelMA lyophilizate can be prepared at various concentrations and photo-crosslinked to yield hydrogels with tunable stiffness. A photoinitiator is required to render the bioink photocrosslinkable and stable for cell culture applications.

Applications

• Low-concentration formulations for droplet/electro-magnetic droplet printheads to generate microspheres.
• High-concentration formulations to create bioinks (e.g., GelMA-based bioinks) or to blend with additives for custom bioinks.
• Compatible with thickeners such as nanofibrillated cellulose, xanthan gum, and glucomannan.
• After 3D cell culture, cells can be harvested for analysis using cell lysis reagents (e.g., Cell Collect G).

Product information

• Contents: Two 500 mg bottles of PhotoGel® 50% DS (sterile, lyophilized GelMA) — package size 1 gram (2 × 500 mg).
• Form: Lyophilized powder.
• Appearance: White to off-white lyophilizate.
• Sterilization method: Filtration; sterility (USP modified): No growth.
• Storage temperature: −20°C.
• Shelf life: Product information lists a minimum 9 months; specification table lists a minimum of 6 months from date of receipt.
• Optional photoinitiators (non-sterile): LAP or Irgacure 2959 (or supplied without photoinitiator).
• Intended use: For research use only. Not for human use.

Publications (examples using PhotoGel® 50% DS)

• Trophoblast Migration with Different Oxygen Levels in a Gel-Patterned Microfluidic System — Ko, Jeon, Kim (2022).
• Bioprinting using PEGDMA-based hydrogel on DLP printer — Chang, Ching, Hashimoto (2022).
• Biomaterial composition and stiffness as decisive properties of 3D bioprinted constructs for type II collagen stimulation — Martyniak, Lokshina, Cruz, et al. (2022).
• Calcium supplementation of bioinks reduces shear stress-induced cell damage during bioprinting — Fischer, Nosratlo, Hast, et al. (2022).
• Optical 4D oxygen mapping of microperfused tissue models with tunable in vivo-like 3D oxygen microenvironments — Wesseler, Johansen, Kiziltay, et al. (2022).

Recommended hardware / compatibility

• Recommended for extrusion bioprinters with temperature-controlled printheads and cooled beds (e.g., BIO X) or heated printheads (e.g., INKREDIBLE+), and for light-based printers such as BIONOVA X.

Technical specifications

• Appearance: White to off-white lyophilizate.
• Form: Lyophilized powder.
• Package size: 1 gram (2 × 500 mg).
• Storage temperature: −20°C.
• Shelf life: Minimum 9 months (product info) / Minimum 6 months from date of receipt (spec table).
• Sterilization method: Filtration.
• Sterility (USP modified): No growth.
• Degree of Methacrylation: 45–55%.
• Viscosity: 168 ± 60 Pa·s.
• pH: 7.0–7.4.
• Source: Type A, 300 Bloom, porcine gelatin.
• Hydrogel Young's Modulus E (Pa): Characteristic (not numerically specified).
• Photoinitiator options: None, Irgacure 2959, LAP.
• Base material: GelMA.
• Properties: Lyophilized.
• Intended use: Biomaterial for bioprinting research; photocuring required for stabilization.