Enzyme reagent Perox Dil. 3

staindiagnosticblood sample

Perox Dil. 3 is used by Technicon H*1, H*2, H*3 hematological analyzer as a whole blood sample DILUTING reagent. SUMMARY AND PRINCIPLE The peroxidase cytochemical reaction consists of 2 steps. In the first step, EDTA anticoagulated whole-blood sample is diluted with Perox Dil. 1 reagent. Surfactants and thermal stress cause lysis of the red blood cells. An aldehyde in Perox Dil.1 reagent fixes the white blood cells. During the second step, Perox Dil. 2 reagent and Perox Dil. 3 reagent are added to the peroxidase reaction chamber. A peroxidase enzyme substrate in Perox Dil. 2 reagent and the hydrogen peroxide in Perox Dil. 3 reagent stain the sites of peroxidase activity in the granules of neutrophils, eosinophils, and monocytes. Lymphocytes, basophils, and large unstained cells contain no granules with peroxidase enzyme activity. A constant volume of the cell suspension from the Perox reaction chamber passes through the flowcell along with Perox Sheath. The two fluids flow as independent, concentric streams (no mixing), with the Perox Sheath stream encasing the sample stream.