Enzyme reagent C002 series

Cas9 Proteinfor mutation detectionliquid

Magigen CRISPR Cas9 protein, spCas9, Streptococcus pyogenes Cas9, is an RNA-guided endonuclease that catalyzes site-specific cleavage of double stranded DNA. It only recognize NGG PAM. SpCas9 makes a blunt cut between the 17th and 18th bases in the target sequence (3 bp 5′ of the PAM). Introduction With its pure protein system that resists distraction and low off-target rate, The CRISPR-Cas9 technologies is a safe and common method in the field of gene editing. Unit Definition ♦ This product is a recombinant protein that carries NLS fragments at both the N- and C-terminals. ♦ This product 1pmol=154 ng. ♦ The design and stabilization of sgRNAs have a great influence on the reaction system, and whether sgRNAs can be degraded by gel electrophoresis before the reaction is carried out. Product Features The RNP system of Cas protein does not required clone construction, no need for vectors and viruses. Its features of low toxicity, quick, efficient, low off-target rates, and able to use in multi-target editing allow it to be used in gene therapy for many diseases such as blood diseases and tumors.