Enzyme reagent Magicscript

reverse transcriptase II plusfor RT-PCRvirus

The Magigen Magicscript RT-II Plus based on the reverse transcriptase (M-MLV Reverse Transcriptase) of Moloney murine leukemia virus (M-MLV) , cloned and expressed by gene recombination technology. The RNase-H activity is completely removed, and reverse transcriptase fragments of different species are chimeric in different structural regions. Introduction The Magigen Magicscript RT-II Plus based on the reverse transcriptase (M-MLV Reverse Transcriptase) of Moloney murine leukemia virus (M-MLV) , cloned and expressed by gene recombination technology. The RNase-H activity is completely removed, and reverse transcriptase fragments of different species are chimeric in different structural regions. Unit Definition One unit is defined as the amount of enzyme that will incorporate 1 nmol of dTTP into acid-insoluble material in 10 minutes at 37°C using poly(rA)•oligo(dT) as template/primer. Product Features - Wider temperature conditions from 37℃ to 65℃, while maintains 100% capabilities around 55℃ constantly. - Strong reverse transcriptase abilities：able to reverse transcript 12kb models that completely removed RNase-H activity. - Comprehensiveness：suitable with mutiple reaction systems like RT-PCR/RT-RDA/RT-LAMP/RT-HDA. Product Performances Set the heat treatment time gradient at 55°C to 0, 50, 100, 150, 200, 250, 300 and 350min, then use Magicscript RT-II Plus at 55°C to start the reverse transcription reaction; with EV71 RNA as a template and proceed with qPCR reaction, the results show that reverse transcriptase II Plus has good thermal stability.