Food safety test kit LSY-20052

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Product overview
The Nitrofuran (AHD) rapid test dipstick is a competitive inhibition immunochromatographic assay for qualitative screening of Nitrofuran (AHD) residues in egg and duck egg samples. The assay uses colloidal gold-labeled monoclonal antibody; presence of AHD in the sample inhibits binding at the test line, producing a visible color difference between test (T) and control (C) lines.

Introduction
Nitrofuran (AHD) rapid test dipsticks for egg matrices. Catalog No. LSY-20052. Kit configured for field or laboratory screening of egg products for regulatory surveillance.

Key points

• Specifications: 96 strips/kit
• Intended use: qualitative detection of Nitrofuran (AHD) in egg and duck egg samples
• Operation: simple workflow suitable for on-site screening; high analytical sensitivity

Detection limit
Egg: 0.5 ng/g (0.5 ppb).

Contents (per kit)

• Nitrofuran (AHD) test dipsticks (test dipsticks, desiccant, micro-wells reagent) — 12 bottles
• Manual — 1 piece
• 1M HCl — 2 bottles
• 1M NaOH — 2 bottles
• 1M K2HPO4 solution — 2 bottles
• Derivatization reagent — 1 bottle
• Sample buffer — 2 bottles

Principle
The assay is based on competitive inhibition immunochromatography: Nitrofuran (AHD) in the sample binds colloidal gold-labeled monoclonal antibody, reducing antibody binding to immobilized Nitrofuran (AHD)-BSA on the test line. The resulting color intensity at the T line relative to the C line provides a qualitative result against the detection limit.

Sample preparation

• Solution preparation: prepare 0.1M K2HPO4 by diluting 1M K2HPO4 with deionized water at 1:9.
• Egg sample treatment:
• 1) Weigh 3 ± 0.05 g homogenized egg sample into a 50 ml centrifuge tube; add 6 ml deionized water, 750 µl 1M HCl and 300 µl derivatization reagent; shake 2 min; incubate at 60 ℃ water bath for 30 min.
• 2) Add 7.5 ml 0.1M K2HPO4, 600 µl 1M NaOH and 10 ml ethyl acetate; shake 1 min; centrifuge at 4000 r/min for 10 min.
• 3) Transfer 5 ml upper organic layer to a glass tube and evaporate to dryness at 56 ℃ under nitrogen or air.
• 4) Add 2 ml n-hexane and shake to dissolve residue completely; add 0.6 ml sample buffer, shake 30 s, allow phase separation; remove upper layer (if emulsion forms, centrifuge 4000 r/min for 5 min).
• 5) Withdraw 200 µl of the lower aqueous layer for testing (avoid aspirating the upper organic layer).

Operation procedures

• 1) Read the manual before use and bring dipsticks and samples to room temperature.
• 2) Prepare required bottles, microwells and dipsticks from the kit; mark samples. Use prepared dipsticks within 1 hour and keep reagent bottles sealed to avoid moisture.
• 3) Add 200 µl of processed sample into a microwell; aspirate and dispense 5–10 times to mix until no visible solids remain.
• 4) Incubate 5 min at room temperature (20–25 ℃), then insert a dipstick with the MAX end fully immersed in the mixture.
• 5) Incubate 5–8 min at room temperature; read result at this time only (other read times are invalid).

Test result interpretation

• Negative: T line is darker than or equal in color to the C line — no Nitrofuran (AHD) residue or below detection limit.
• Positive: T line is obviously lighter than the C line or T line is absent — Nitrofuran (AHD) residue at or above detection limit.
• Invalid: C line absent — test invalid (expired or incorrect operation); repeat with a new test.

Precautions

• Use each test strip once at room temperature; do not use after expiry date.
• Avoid reuse of pipette tips to prevent cross-contamination.
• Do not touch the central white membrane of the test strip; avoid direct sunlight and strong airflow during testing.
• Wear appropriate personal protective equipment (gloves, lab coat) when handling reagents and samples.

Specificity
No cross-reaction observed with Nitrofuran AMOZ, AOZ or SEM under the stated conditions.

Storage and expiry
Store at 2–8 ℃ in a sealed, dry and dark place; do not freeze. Expiry: 12 months (production date printed on box).

Caractéristiques / spécifications techniques

• Product name: Nitrofuran (AHD) rapid test dipsticks (Egg)
• Catalog number: LSY-20052
• Kit size: 96 strips/kit
• Contents: test dipsticks, desiccant, micro-wells reagent, manual, 1M HCl, 1M NaOH, 1M K2HPO4, derivatization reagent, sample buffer
• Detection limit (egg): 0.5 ng/g (0.5 ppb)
• Principle: competitive inhibition immuno-chromatography
• Storage conditions: 2–8 ℃, sealed, dry, dark
• Expiry: 12 months
• Specificity: no cross reaction with AMOZ, AOZ, SEM