Food safety test kit LSY-20044

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Product Overview
The β-lactam antibiotics rapid test dipstick LSY-20044 is a colloidal gold immunochromatographic strip for qualitative on-site screening of β-lactam residues in cow and goat raw milk. The test provides a visual comparison between the T (test) line and the C (control) line to indicate presence of residues.

Introduction
Simple, single-use dipstick designed for rapid field testing of β-lactam antibiotic residues in milk from dairy animals. Intended for preliminary screening in farms, milk collection centers and quality control labs in the agricultural sector.

Packaging & Contents

• Each bottle: 8 microwells + 8 dipsticks.
• Kit: 12 bottles per kit.
• Instruction leaflet: 1 piece included.

Principle
The test uses a competitive inhibition immuno-chromatographic assay: when β-lactam residues in the sample reach or exceed the detection limit, they bind the labeled antibody and reduce the signal at the T line versus the C line. The C line must be visible for a valid result.

Detection limits (ppb)

• Penicillin G: 2–4
• Amoxicillin: 2–3
• Ampicillin: 2–3
• Oxacillin: 5–7
• Cloxacillin: 4–6
• Piperacillin: 6–8
• Nafcillin: 15–25
• Dicloxacillin: 6–8
• Cefquinome: 8–15
• Cefalotin: 30–40
• Cefazolin: 40–50
• Cephalonium: 8–10
• Cefacetrile: 20–30
• Cefoperazone: 3–5
• Cephapirin: 8–10
• Ceftiofur: 60–100

Operation procedures

• Read the instruction leaflet before testing. Bring dipsticks and milk sample to room temperature.
• Absorb 200 µL milk into the micro-well. Mix by pipetting or sucking 5–10 times until homogeneous and free of solids.
• Incubate the sample at 40 °C for step 1 (recommended). If no incubator available, room temperature (≈25 °C) is acceptable. Time: 3 min.
• Insert the dipstick with the end marked MAX downward. Incubate at 40 °C for step 2 (recommended) or at room temperature if necessary. Time: 3 min (extend by 1 min for high-fat samples).
• Remove the dipstick and interpret visually by comparing T and C lines.

Test Result Interpretation

• T line darker than C line — Negative: β-lactam residue is below detection limit.
• T line equal to C line — Weak positive: β-lactam residue approximately at detection limit.
• T line lighter than C line or T line absent — Positive: β-lactam residue above detection limit.
• C line absent — Invalid: test failed, repeat with a new dipstick.

Precautions

• For raw cow and goat milk only. Not suitable for processed or heavily adulterated samples.
• Use dipsticks and micro-wells only once; do not use expired tests.
• After removing dipsticks, reseal the bottle to protect remaining strips from humidity and light.
• Avoid touching the white membrane. Protect from direct sunlight and strong airflow during storage and testing.
• Store samples refrigerated and test within 3 days. Ensure milk is fully liquid, mixed and free of precipitation before testing.
• For high-fat samples, chromatography may slow; extend step 2 by 1 minute if recommended.
• Confirm positive results by repeat testing or laboratory reference methods before making regulatory decisions.

Specificity
No cross-reaction observed with tetracyclines, quinolones, sulfonamides, chloramphenicol, melamine and similar common adulterants.

Storage and Expiry
Store sealed in a dry, dark place at 2–8 °C; do not freeze. Shelf life: 12 months. Production date printed on the box.

Technical specifications

• Product name: β-lactam antibiotics rapid test (Milk)
• Catalogue No.: LSY-20044
• Intended matrices: cow milk and goat milk (raw milk)
• Assay format: colloidal gold immunochromatographic dipstick
• Packaging: 8 microwells + 8 dipsticks per bottle; 12 bottles per kit
• Instruction: 1 leaflet included
• Recommended incubation: two steps at 40 °C, 3 min each (room temperature acceptable without incubator)
• Detection limits: see Detection limits block above (ppb)
• Result readout: visual comparison of T line vs C line
• Specificity: no cross-reaction with listed classes of compounds
• Storage: 2–8 °C, sealed, dry, dark; shelf life 12 months