OverviewAcid Alcohol is a laboratory differentiating solution used in histology and cytology to remove excess hematoxylin and to sharpen nuclear detail after hematoxylin staining for H&E and PAP protocols. Formulated with either 0.5% or 1% hydrochloric acid in 70% reagent-grade ethanol, it supports controlled differentiation to improve reproducibility of staining results.
Key features and intended use- Removes excess hematoxylin and defines nuclei following hematoxylin staining.
- Designed for routine H&E and PAP staining workflows in histology/cytology labs.
- Available in controlled formulations (0.5% and 1% HCl) to match differentiation requirements.
- Helps standardize staining outcomes and enhance nuclear contrast for microscopic evaluation.
- For professional laboratory use only; follow IFU and SDS for handling and safety.
Available variants / pack sizes- 0.5% Acid Alcohol — 5 litres (SKU: 3803650E)
- 1% Acid Alcohol — 1 gallon (SKU: 3803650)
Country availability- Available through regional distributors and direct channels in multiple countries worldwide. Contact your local distributor or Leica Biosystems representative for exact availability and ordering details.
Documents provided (titles)- IFU: differentiator_rev_a.pdf
- SDS (0.5%): Acid_Alcohol_0.5_AU.pdf
- SDS (1%): Acid_Alcohol_1_EN.pdf
- SDS (1% AU): Acid_Alcohol_1__AU_1.2.pdf
Technical characteristics / specifications- Commercial name: Acid Alcohol
- Formulations: 0.5% HCl in 70% reagent-grade alcohol; 1% HCl in 70% reagent-grade alcohol
- Intended use: Differentiation after hematoxylin staining for H&E and PAP methods; removal of excess stain and definition of nuclei
- Pack sizes / SKUs: 0.5% — 5 litres (SKU 3803650E); 1% — 1 gallon (SKU 3803650)
- Included documentation: IFU and SDS for applicable strengths
- Brand: Leica Biosystems