Automated cell imaging system Viventis SCAPE

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Overview
Escape the limits of 3D live-cell imaging with single-objective light sheet microscopy. The Viventis SCAPE system uses swept confocally aligned planar excitation (SCAPE) to deliver gentle, fast volumetric imaging directly from standard flat sample carriers.

Key benefits

• Fast volumetric imaging without moving the sample using SCAPE scanning, enabling real-time tracking of rapid signaling and higher throughput acquisitions.
• Reduced phototoxicity and photobleaching by illuminating only the imaging plane, permitting fast or long-term volumetric imaging of light-sensitive specimens.
• No special sample mounting required: inverted configuration and oblique plane illumination (OPM) allow imaging of standard flat-bottom carriers and multiwell plates (supports at least 96-well plates).
• Versatile sample compatibility: 2D adherent cultures, tissue slices, 3D cultures, larger embryos and small model organisms; large fields of view achievable by tiled acquisitions.

Image quality and acquisition features

• Auto-alignment for optimized light sheet settings with a single click to obtain sharp, high-resolution volumes.
• Beam pivoting to reduce stripe artifacts from scattering for cleaner images.
• On-the-fly deskewing (Slice of Life) to convert the oblique SCAPE view into a conventional xyz perspective for intuitive navigation and immediate analysis.
• SCAPE-optimized computational clearing and LIGHTNING processing for enhanced contrast and improved resolution.
• Example imaging speed: 1.5 s/volume (reported for an example U2OS acquisition).

Throughput and workflow

• Supports higher throughput imaging by capturing more samples in less time; compatible with multi-position experiments and multiwell plate workflows.
• Complete workflow support including Leica service and application experts, multi-level training programs, regional logistics for genuine parts, and Leica OneCall application support.

Applications

• 2D adherent cell cultures
• Tissue slices
• 3D cell cultures and spheroids
• Larger embryos and small model organisms (e.g., C. elegans timelapse examples)

Technical specifications

• Imaging principle: SCAPE (swept confocally aligned planar excitation) / single-objective light sheet
• Sample compatibility: standard flat-bottom carriers and multiwell plates (supports at least 96 wells)
• Objective working distance: determines accessible z-volume (example cited: 620 µm working distance for objective limits)
• Field of view: x-dimension limited by stage travel range; large fields achieved by tiled acquisitions
• Image enhancement: auto-alignment, beam pivoting, computational clearing, LIGHTNING
• Realtime processing: on-the-fly deskewing (Slice of Life) and reslicing
• Demonstrated imaging speed example: 1.5 seconds per volume (U2OS example)
• Supported sample types: 2D cells, tissue slices, 3D cultures, embryos, small organisms
• Workflow support: Leica service & applications team, Leica training (multi-level factory certification), regional logistics hubs for parts, Leica OneCall application support