Solution reagent kit diarellaGBS

diagnosticfor DNA qualificationfor RT-PCR

The diarellaGBS real time PCR is an assay for the detection of the DNA of GBS in clinical specimens. 2 Pathogen Information Streptococci mainly colonise mucous membranes in humans. Beta-hemolyzing streptococci of serogroup B (GBS) in the maternal genital tract can be transmitted to the child during birth. The Transmission may lead to early onset illness within the first hours up to 3 days after birth with serious systemic infection and pneumonia. Aetiopathology with shock symptoms and neurologic long-term damages may occur. Late onset illness can be cuased by transmission by the mother or e.g. nursing staff. Early onset infection occurs within app. 1 of 2.000 newborns. The affected majority (app. 80 %) are mature newborns. App. 4 % of the diseased children die. The following factors promote the transmission of GBS: •Presence of GBS in high titers in the maternal genital tract at birth. •Period of more than 18 h between rupture of membranes and confinement. •Maternal fever during birth. •Preterm birth before week 37 of pregnancy. •GBS present in maternal urine during pregnancy. •Previous birth of a child with GBS infection. In order to minimize the risk of GBS-transmission to newborns, pregnant women should be examined between week 35 and 37 of pregnancy. In case of a GBS-positive result, antibiotics treatment of the mother during birth can be done. In case of a negative result within 5 weeks before birth, antibiotics treatment of the mother can be avoided. 3 Principle of the Test The diarellaGBS real time PCR contains specific primers and dual-labeled probes for the amplification and detection of GBS DNA in clinical specimens.