Solution reagent kit diarellaBabesia

diagnosticfor RT-PCRfor DNA qualification

The diarellaBabesia real-time PCR is an assay for the detection of DNA of Babesia divergens, Babesia microti and Babesia sp. EU1 (Babesia venatorum), extracted from clinical specimens and ticks. 2 Pathogen Information Babesiosis is a malaria-like parasitic disease caused by infection with Babesia, a genus of protozoal piroplasm. The parasites are transmitted by ticks such as dermacentor reticulatus, a hardbodied tick nowadays common throughout Central Europe. Dependent on the species, the incubation time varies between 7 to 20 days. Rupture of the erythrocytes causes anaemia. Infections can remain without symptoms or with mild fever and diarrhea. However, in more severe cases, high fever with chills can occur. In Europe infections are mainly caused by B. divergens and B. sp. EU1, while in the US B. microti (East Coast and Mid-West) and B. duncani (North-West Coast) are the causative agents for human babesiosis. 3 Principle of the Test diarellaBabesia contains specific primers and dual-labeled probes for the amplification and detection of Babesia DNA, extracted from clinical specimens and ticks. The presence of nucleic acid is detected by an increase in fluorescence due to hydrolysis of the probes during amplification. The fluorescence of the pathogen-specific probes is measured in the FAM channel. Furthermore, diarellaBabesia contains a Control DNA, which is added during DNA extraction and detected in the same reaction by a differently labeled probe. The Control DNA allows the detection of PCR inhibition and acts as control, that the nucleic acid was isolated from the clinical specimen.