The virellaNoro real time RT-PCR TM is an assay for the detection of Norovirus RNA (Genogroup I and II) in stool, food and environmental samples using real time PCR microplate systems. The virellaNoro real time RT-PCR Kit TM detects Norovirus strains of high genetic diversity, such as the following:
GI: Norwalk, Desert Shield, Winchester, Queensarms, Southhampton, Chiba
GII: Lordsdale, Bristol, Melksham, Toronto, Hawaii
2 Pathogen Information
Noroviruses are small non-enveloped RNA viruses belonging to the family of Caliciviridae. They cause approximately 90 % of epidemic non-bacterial outbreaks of gastroenteritis around the world.
The viruses are transmitted by fecally contaminated food or water and by person-to-person contact. For this reason, outbreaks of Norovirus infection often occur in closed or semi-closed communities, such as long-term care facilities, hospitals, prisons, dormitories, and cruise ships.
Noroviruses are highly contagious and are stable at temperatures between -20°C to +60°C and in acidic environments up to pH 3.
Norovirus infections occur throughout the year, however, in Europe, seasonal increases are observed between October and March.
The virellaNoro real time RT-PCR Kit TM detects Norovirus strains of high genetic diversity, such as the following:
GI: Norwalk, Desert Shield, Winchester, Queensarms, Southhampton, Chiba
GII: Lordsdale, Bristol, Melksham, Toronto, Hawaii
3 Principle of the Test
The virellaNoro real time RT-PCR Kit TM contains specific primers and duallabeled probes for the amplification and detection of Norovirus RNA (GI and GII) in stool,