This kit can be used in two modes. It can be used for isoform discovery or to evaluate transcript adundance. Running it in the first mode will deliver abundance data as well. This kit provides a comprehensive survey and accurate relative abundance of every transcript in RNA samples. Single molecule long read data is generated as well as relative abundance using barcode based molecular counting. It also features a multiplex workflow that pools 24 samples into a single tube with less than 3 hours of hands-on time.
It’s the perfect kit for labs looking to profile every transcript in their sample. Compatible with all Illumina instruments.*
*HiSeqs, NextSeqs, and NovaSeqs are recommended for per base cost benefits.
Mechanism of Action
Synthetic long read sequencing
Allows for 8 samples to be processed in one tube
10ng of total RNA
HiSeq 2500, HiSeq 3000, HiSeq 4000, NextSeq, NovaSeq, MiSeq, MiniSeq
Nucleic Acid Type
2 x 150 Paired End (PE) sequencing
Specialized Sample Types
Assembly of a synthetic long reads form short reads
The advantages of long-read sequencing. the convenience
of your existing sequencing infrastructure
Next generation sequencing (NGS) has been an incredibly powerful technique, but there
are many applications where short-read sequencing comes up, well, short.
Fortunately, Loop Genomics has developed an affordable, easy-to-implement solution that uses existing
Illumina NGS sequencers to produce full-length, long-read sequences.