Solution reagent kit LoopSeq™ Transcriptome

NGSfor DNA sequencingfor molecular biology

This kit can be used in two modes. It can be used for isoform discovery or to evaluate transcript adundance. Running it in the first mode will deliver abundance data as well. This kit provides a comprehensive survey and accurate relative abundance of every transcript in RNA samples. Single molecule long read data is generated as well as relative abundance using barcode based molecular counting. It also features a multiplex workflow that pools 24 samples into a single tube with less than 3 hours of hands-on time. It’s the perfect kit for labs looking to profile every transcript in their sample. Compatible with all Illumina instruments.* *HiSeqs, NextSeqs, and NovaSeqs are recommended for per base cost benefits. Assay Time 8.5 hours Hands-On Time 2.5 hours Mechanism of Action Synthetic long read sequencing Multiplexing Allows for 8 samples to be processed in one tube Input Quantity 10ng of total RNA Category Transcriptome System Compatibility HiSeq 2500, HiSeq 3000, HiSeq 4000, NextSeq, NovaSeq, MiSeq, MiniSeq Nucleic Acid Type RNA Method 2 x 150 Paired End (PE) sequencing Specialized Sample Types Eukaryotic sample Technology Assembly of a synthetic long reads form short reads Automation Capability Yes The advantages of long-read sequencing. the convenience of your existing sequencing infrastructure Next generation sequencing (NGS) has been an incredibly powerful technique, but there are many applications where short-read sequencing comes up, well, short. Fortunately, Loop Genomics has developed an affordable, easy-to-implement solution that uses existing Illumina NGS sequencers to produce full-length, long-read sequences.