Cytopathic effect rapid test 501010

rotavirusfecesfecal

INTRODUCTION Rotavirus is the most common agent responsible for acute gastroenteritis, mainly in young children. Its discovery in 1973 and its association with infantile gastro-enteritis represented a very important advancement in the study of gastroenteritis not caused by acute bacterial infection. Rotavirus is transmitted by oral-fecal route with an incubation period of 1-3 days. Although specimens collected within the second and fifth day of the illness are ideal for antigen detection, rotavirus may still be found while diarrhea continues. Rotaviral gastroenteritis may result in mortality for populations at risk such as infants, the elderly and immunocompromised patients. In temperate climates, rotavirus infections occur mainly in the winter months. Endemics as well as epidemics affecting some thousand people have been reported. With hospitalized children suffering from acute enteric disease, up to 50% of the analyzed specimens were positive for rotavirus. The viruses replicate in the cell nucleus and tend to be host species-specific producing a characteristic cytopathic effect (CPE). Because rotavirus is extremely difficult to culture, it is unusual to use isolation of the virus in the diagnosis of infections. Instead, a variety of techniques have been developed to detect rotavirus in feces. PRINCIPLE The Rotavirus Rapid Test Device (Feces) detects rotavirus through visual interpretation of color development on the internal strip. Anti-rotavirus antibodies are immobilized on the test region of the membrane. During testing, the specimen reacts with anti-rotavirus antibodies conjugated to colored particles and precoated onto the sample pad of the test.