cylindrical collection tube
LIND-VAC WE1102, WE3102
A vacuum tube is designed to collect venous blood for the subsequent analysis of free extracellular DNA. This kind of analysis is necessary for examination of DNA fragments in the plasma.
These can be fragments of fetal DNA in the blood of the mother. Its analysis lets determine the gender of the child at the early stages of development and identify some hereditary mutations.
Also among free extracellular DNA there may be DNA fragments with somatic mutations resulting from carcinogenesis. Analysis of these fragments (liquid biopsy) allows to diagnose the presence of malignant neoplasms at the early stages.
Another area of application of the test tube is hematological or genetic studies that require a long-term storage of blood.
The principle of operation is based on additional stabilization of cell membranes and extracellular DNA and on the maximum inhibition of cellular metabolism using such preservative agents as aminocaproic acid, glycine, inositol.
At the preanalytical phase for blood collection can be used standard vacuum systems. After venipuncture, the tube must be gently inverted several times to better mix the sample with the additive sprayed on the tube’s walls.
Normally for further analysis, centrifugation at a speed of 2000-6000 G is necessary. The speed of centrifugation depends on a type of the DNA isolation kit used. As a result, complete separation of the cellular components of blood and plasma containing extracellular DNA is achieved. Extracellular DNA stabilization is possible for a period of 7-14 days at a storage temperature of 4-30 C.