COVID-19 assay kit FastPlex™

SARS-COV-2nasalthroat

PreciGenome FastPlex Triplex SARS-CoV-2 Detection Kit is a fast, highly sensitive multiplex diagnostic solution. It consists of both the assays and controls, which are needed for the real-time PCR RNA detection  from the SARS-CoV-2 virus. It is a multiplex RT-PCR diagnostic kit, enabling quick and precise diagnosis of COVID-19 caused by SARS-CoV-2 infection. The 1 step SARS-CoV-2 detection kit is based on the PCR method which uses a fluorescent probe and a specific primer to detect two specific regions within the novel coronavirus (SARS-CoV-2) nucleocapsid protein N gene. This molecular panel aids in the detection of viral RNA from SARS-CoV-2, the causative agent of COVID-19. The kit is used with Rainamp™ VTM with proprietary releasing agent which enables the user to use crude Nasal or throat swabs directly in their PCR reactions. By simply mixing the Rainamp VTM with the sample, the viral RNA is immediately stabilized, and can be released efficiently with extraction step. This eliminates the need for laborious, costly RNA extraction and purification steps, drastically improving upon the traditional workflow of PCR, while removing potential sample loss and contamination. This kit uses the 2019-Novel Coronavirus (Covid-19) ORF 1ab and the specific conserved sequence encoding the nucleocapsid protein N gene as the target regions to carry out the dual-target genes design. A one-step reverse transcription-polymerase chain reaction (RT-PCR) combined with Taqman hydrolysis probe technology was used to detect the RNAs in the sample by a fluorescence quantitative PCR instrument with changes in fluorescence signal.