Intact, Amplifiable DNA from FFPE Tissue
Minimal preparation time: simply add ethanol and go!
Safely deparaffinize without using harsh organic solvents
No overnight digestion required
Higher quality DNA from FFPE samples
ReliaPrep™ FFPE gDNA Miniprep System provides a convenient method for purifying genomic DNA from formalin-fixed paraffin-embedded (FFPE) tissues. Extraction of DNA from FFPE tissues is challenging because the formalin fixation process results in cross-linking between proteins and DNA, as well as between different strands of DNA. This nonoptimal preservation of genomic DNA in FFPE complicates its use in many standard downstream analysis applications.
The ReliaPrep™ FFPE gDNA Miniprep chemistry is optimized to partially reverse this cross-linking without the need for an overnight digestion. The protocol also incorporates a deparaffinization method that does not use xylene or other hazardous or volatile solvents.
The protocol is complete in 2.5 hours with minimal hands-on time.