Dye reagent kit QuantiFluor® ONE

for microbiologyfor nucleic acids

Specific quantitation of dsDNA Increased sensitivity over absorbance at 260nm for low-concentration samples Simple to use and automation-friendly Simple, Accurate DNA Quantitation. No Dilutions. No Extra Tubes. The QuantiFluor® ONE dsDNA System provides a fluorescent double-stranded DNA-binding dye (504nmEx/531nmEm) in an automation-friendly, "Add-and-Read" format for both dye and standard, simplifying DNA quantitation and speeding up your workflow. It's as easy to use as NanoDrop® absorbance-based methods, but much more sensitive for low-concentration samples. Improved Sensitivity vs. Absorbance and Other dsDNA Fluorescent Quantitation Systems The QuantiFluor® dsDNA dye offers greater sensitivity over absorbance at 260nm (NanoDrop) low-concentration samples, and better or equal sensitivity performance when compared to Quant-IT™ dsDNA BR. QuantiFluor® ONE dye sensitivity range is 0.2–400ng/µl (1µl sample in 200µl assay). DNA Quantitation Assay Sensitivity and Linearity The QuantiFluor® ONE System detects as little as 1ng/ml dsDNA per assay using the Quantus™ Fluorometer (corresponding to 1µl of a 200ng/ml starting sample). Detection limit is defined as greater than three standard deviations above background RFU. These graphs show representative QuantiFluor® ONE dsDNA System standard curves generated from Lambda or K562 DNA standards in a 96-well plate format. The assay linearity and dynamic range of either the QuantiFluor® ONE Lambda DNA or Genomic K562 DNA standards are shown from a range of 0.2–400ng/µl (1µl sample in a 200µl assay).