The purification of small RNA species such as microRNA (miRNA) is challenging because of the low concentrations, small fragment sizes (19-22 nucleotides) and tendency to be found in protein complexes. Due to its essential role in homeostasis, miRNA is an important biomarker for cancer and metabolic disease research.
With only minimal hands-on time, the chemagic miRNA kits enable high throughput and efficient extraction of circulating cell-free small RNA from 200 µl or 1 ml inputs in 24-well or 96-well format. The chemagic miRNA kits obtained equivalent yields to manual methods, while analysis by quantitative PCR and Next Generation Sequencing revealed high diversity and reliable performance.
Overview
Specifications
The chemagic miRNA 200 Kit H96 is designed for extracting small RNA (microRNA) from up to 200 µl plasma, serum, or saliva samples, and extracellular vesicles on the chemagic 360 instrument equipped with a96 Rod Head. It offers consistent and scalable extraction with comparable yields to manual methods, making it suitable for various downstream applications like biomarker discovery and gene expression analysis.
Specifications
Format 24 Rod Head Automated
Genomic Analysis Oncology
Storage Conditions Room Temperature
Technology M-PVA Magnetic Bead Technology
Throughput Up to 96 samples per run
Unit Size 960 preps/kit