The purification of small RNA species such as microRNA (miRNA) is challenging because of the low concentrations, small fragment sizes (19-22 nucleotides) and tendency to be found in protein complexes. Due to its essential role in homeostasis, miRNA is an important biomarker for cancer and metabolic disease research.
With only 10 minutes of hands-on time within a 90-minute total processing time, the chemagic miRNA kits enable high throughput and efficient extraction of circulating cell-free small RNA from 200 µl or 1 ml inputs in 24-well or 96-well format. The chemagic miRNA kits obtained equivalent yields to manual methods, while analysis by quantitative PCR and Next Generation Sequencing revealed high diversity and reliable performance.
Overview
The chemagic miRNA 1k Kit H24 is designed for the purification of circulating small RNA (microRNA) from 1 ml plasma, serum, or saliva samples, as well as from extracellular vesicles on the chemagic 360 instrument equipped with 24 Rod Head. The kit is designed for consistent and scalable small RNA extraction with comparable yields to manual methods, as well as suitability for various downstream assays.
Specifications
Other specifications
Storage Conditions Room Temperature
Technology M-PVA Magnetic Bead Technology
Throughput Up to 24 samples per run
Unit Size 240 preps/kit
Format
24 Rod Head
Automated