Solution reagent kit HOT FIREPol® MultiPlex Mix

DNA polymeraseTaq DNA polymerasefor microbiology

Hot-start master mix for high throughput applications. Analyze multiple targets per reaction. Increased sensitivity and yield. Prevents primer dimer formation. Reaction set-up and shipment without dry ice. HOT FIREPol® MultiPlex Mix is a premixed ready-to-use solution containing all reagents required for hot-start multiplex PCR (except template, primers, and water). Generated PCR products are compatible with TA cloning procedures. Properties Concentration: 5x Hot-start: yes, initial activation in 12-15 min Ready to load: no Fidelity: 1 x Taq Cloning Type: T/A cloning Multiplexing: up to 18 targets (depending on amplicon length) Amplicon Size: up to 5 kb Mix Components HOT FIREPol® DNA polymerase 5x Multiplex Buffer with 10 mM MgCl2 (1x PCR solution – 2 mM MgCl2) dNTPs BSA Compound that increases sample density (loading dyes not included)